Jack Crow, a relentless and tough-as-nails vampire hunter, leads a team known for exterminating the undead. Their latest mission results in the destruction of a nest in New Mexico, but the celebration is cut short when they fall victim to a surprise attack by Valek, a centuries-old master vampire. Nearly all of Jack's team is slaughtered, and the vampire-hunting operation's backer, Cardinal Alba, is none too pleased. Determined to get revenge and prevent an impending vampire apocalypse orchestrated by Valek, Jack must regroup and strategize for what's likely to be his toughest battle yet.

Injured and desperate, Jack enlists the help of Montoya, one of his few remaining allies, and Katrina, a recently bitten prostitute who develops psychic links to Valek. This connection becomes crucial as it allows them to track the ancient vampire's movements and anticipate his next moves. The team discovers that Valek seeks a powerful relic that could grant him invincibility and the ability to walk in daylight. Armed with this knowledge, Jack races against time and uncovers dark secrets about his own organization and its links to the vampire menace. The narrative intensifies with each confrontation as he closes in on his enemy, facing moral dilemmas and personal demons along the journey.