Tick, a Sydney-based drag performer, accepts an invitation to perform at a resort in Alice Springs, Australia. He recruits his transgender friend, Bernadette, and young, flamboyant fellow drag artist Adam for the journey across the Outback. Together, they board a bus they affectionately name "Priscilla." The trio set off on their adventure, eager to leave behind their troubles and embrace the open road. Along the way, they experience the challenges and joys of their unique friendship and individual identities, facing both acceptance and prejudice from the various small towns they visit.

At each stop, the group encounters different characters that influence their journey and dynamics. The relationships within the trio are tested as secrets and personal struggles come to light, requiring them to support one another in unexpected ways. Their journey brings a mix of comical mishaps and poignant moments, highlighting themes of acceptance, personal growth, and the courage to be true to oneself. With vibrant costumes and performances, they bring joy to the communities they visit while also confronting and overcoming social barriers. The journey ultimately becomes one of self-discovery, acceptance, and the affirmation of their identities.