Pee-wee Herman is an eccentric, child-like man living a whimsical life in his colorful home, cherishing his beloved red bike above all else. His pride and joy is stolen, leading him to go on an adventure across the country to retrieve it. Fueled by a fortune teller's cryptic clue, Pee-wee's journey takes him through quirky locales and introduces him to a myriad of oddball characters, from a friendly waitress to a circus performer. His encounters are filled with slapstick humor, bizarre events, and his infectious enthusiasm.

Throughout his travels, Pee-wee faces numerous challenges and setbacks but remains steadfast in his quest to find his bike. He escapes danger, performs impromptu acts, and experiences unexpected heartwarming moments. Each step of the way, his pure-hearted determination keeps him moving forward, despite the growing absurdity of his situation. He ultimately discovers that his true adventure lies not just in the retrieval of his bike, but in the friendships and life lessons he gains along the way.