Basketball superstar LeBron James finds himself at odds with his youngest son, Dom, who dreams of becoming a video game developer rather than following his father's footsteps on the court. LeBron's attempts to guide Dom into basketball lead to a father-son rift. When a visit to Warner Bros. studios ends with LeBron and Dom trapped in the "Serververse," a digital space controlled by a rogue AI named Al-G Rhythm, the stakes are raised. Al-G seeks validation and wider recognition, feeling overlooked despite running the entire Serververse, and he challenges LeBron to a high-stakes basketball game to earn his and his son's freedom.

LeBron must recruit a team from the residents of the Serververse to face Al-G's digitized champions, the "Goon Squad." He aims for the heroes of the Warner Bros. catalog but is sidetracked to the Looney Tunes world instead. Relegated to the forgotten area of the Serververse, the Looney Tunes have dispersed across various Warner worlds. LeBron must convince a disparate Bugs Bunny and the rest of the Tune Squad to join him on the court, merging their animated zaniness with his basketball prowess. Together, they need to find a way to work as a team despite their vastly different approaches to life and the game to challenge Al-G's formidable line-up.