In the depths of a secret underground research facility known as The Hive, operated by the megacorporation Umbrella, a mysterious theft triggers a catastrophic event. A vial containing a potent viral agent is stolen and released into the facility's air circulation system, swiftly transforming the staff into aggressive and lethal undead creatures. To contain the threat, the facility's artificial intelligence, known as the Red Queen, seals The Hive and kills everyone inside. Nearby, Alice wakes up in a deserted mansion with amnesia, unable to recall her identity or her purpose there. Soon, she is joined by an elite military unit dispatched by Umbrella to investigate and shut down the Red Queen.

The team penetrates The Hive through the mansion, which conceals secret entrances to the facility. As they advance, they are confronted by a variety of security defenses, including laser grids and the monstrous results of Umbrella's unethical bioengineering research. Alice gradually regains fragments of her memory, including her professional expertise, which demonstrates that she is more than she seems. As the soldiers battle the undead and the horrors within The Hive, they must quickly uncover the truth about the viral outbreak to stop it before it spreads to the surface. Trust among them wanes as the stake of the mission goes beyond mere survival, pushing them to make harrowing decisions in an atmosphere rife with paranoia and imminent danger.