Four years after the destruction of Isla Nublar, dinosaurs now roam the earth, living and hunting alongside humans all over the world. This fragile balance will reshape the future and determine, once and for all, whether human beings are to remain the apex predators on a planet they now share with history's most fearsome creatures. Owen Grady and Claire Dearing, former Jurassic World employees, have settled in a remote cabin in the Sierra Nevada mountains, where they are raising Maisie Lockwood, the cloned granddaughter of Jurassic Park's original financial backer. Maisie, now a teenager, struggles with her identity and the implications of her existence. Meanwhile, Blue, the highly intelligent Velociraptor raised by Owen, has given birth to a baby, Beta. Owen and Claire live a quietly vigilant life, keeping Maisie and Beta hidden from those who wish to exploit them.

Across the globe, the ecological disturbance has led to a dramatic increase in dangerous encounters between humans and dinosaurs. In response, a new company called Biosyn Genetics, led by CEO Lewis Dodgson, has established a sanctuary in Italy's Dolomite Mountains, supposedly to study and protect the dinosaurs, collecting their DNA for unknown purposes. Dr. Ellie Sattler observes an unusual phenomenon involving giant locusts, whose unchecked swarms are devastating crops and threatening the global food supply. Suspecting a link between Biosyn and the locust outbreaks, she seeks the help of her college friend, Dr. Alan Grant. Together, they embark on a quest to uncover the truth, leading them to a consequential reunion with another old friend, chaos theory mathematician Dr. Ian Malcolm, who is now associated with Biosyn.