During the American Civil War, the film follows the lives of the four March sisters—Jo, Meg, Beth, and Amy—as they grow up and navigate the challenges of their changing world. Their mother, Marmee, holds the family together while their father is away fighting in the war. The sisters each have distinct personalities: Jo is a headstrong, aspiring writer; Meg is mature and responsible; Beth is shy and musically talented; and Amy is artistic and yearns for a sophisticated life. Despite their differing aspirations, the sisters share a close bond that supports them through trials and joys. They form friendships with their wealthy neighbor, Theodore "Laurie" Laurence, who becomes an extended part of their family. Throughout the narrative, the March sisters face social pressures, personal ambitions, and romantic entanglements, as they strive to find their places in a society that offers limited roles for women.

Jo's determined spirit leads her to pursue an independent life as a writer, challenging the traditional expectations placed upon her. Meg grapples with her desires for both material comfort and genuine affection. Beth's gentle nature endears her to everyone she meets, but she faces her own fragile health. Amy dreams of grandeur but learns important lessons about love and artistry. The film explores themes of sisterhood, resilience, and self-discovery, painting a vivid picture of their coming-of-age journeys as they learn what truly matters in life. As they support each other and evolve, the sisters confront the realities of loss, love, and the importance of remaining true to themselves without compromising their values.