Barbie, along with her sisters Skipper, Stacie, and Chelsea, returns to their hometown of Willows to spend the summer with their grandmother. While rummaging through the attic, they uncover a long-lost treasure map that belonged to their great-grandmother. Excited by the prospect of an adventure, the sisters set off with their puppies—Honey, DJ, Rookie, and Taffy—to find the hidden treasure. Each sister and her puppy bring their unique abilities to the quest, solving riddles and overcoming various obstacles as they explore the town and its surrounding areas.

As they follow the map's clues, they encounter old friends and make new ones, learning valuable lessons about the importance of teamwork, family, and perseverance. Each discovery brings them one step closer to the treasure, but they also face challenges that test their determination and creativity. The journey is filled with fun, mystery, and bonding moments that strengthen their relationships. Through their adventure, Barbie and her sisters learn that the true treasure lies not in gold or jewels but in the memories and experiences shared together.