Two years after his escape from the covert Treadstone program, Jason Bourne is living a low-profile life in India with his girlfriend Marie. Despite suffering from fragmented memories of his past as a highly-trained assassin, Bourne is determined to leave that world behind. Their relative peace is shattered when a failed assassination attempt on Bourne drags him back into the world of espionage. Mistakenly identified as the culprit in a high-profile CIA operation gone awry, Bourne is forced to go on the run once again in a bid to clear his name.

Pursued by relentless CIA operatives, Bourne travels across various international locations to uncover the truth. At the same time, he has to grapple with flashes of his past that keep surfacing, offering him clues about his true identity and the origins of his skills. Meanwhile, CIA Deputy Director Pamela Landy is leading the hunt for Bourne, delving into the files of the defunct Treadstone, and beginning to unravel a deeper conspiracy. As Bourne edges closer to piecing together the disjointed memories of his past, he realizes that he is not just fighting for survival but also for understanding the reasons behind his involvement in a concealed battle between shadowy figures in the intelligence community.