In Pixie Hollow, a dust-keeper fairy named Zarina becomes fascinated with the properties of blue pixie dust, which has the ability to enhance the magical capabilities of regular pixie dust. Her unauthorized experiments lead to disastrous results, causing her to be stripped of her duties. Upset, Zarina leaves Pixie Hollow and joins a group of pirates. A year later, during the Four Seasons Festival, she returns and steals the blue pixie dust. Tinker Bell and her friends, including Silvermist, Rosetta, Vidia, Fawn, and Iridessa, give chase to retrieve it and discover that Zarina has become the captain of a pirate ship.

The fairies' pursuit leads them to face numerous challenges and use their unique talents to navigate Zarina’s traps. Along the way, they contend with their abilities being switched due to Zarina’s experiments with pixie dust. The journey is filled with peril and heartwarming moments as Tinker Bell tries to reason with Zarina and understand her motivations. Ultimately, their friendship and teamwork will determine whether they can bring Zarina back and restore order to Pixie Hollow before it's too late.