The story follows a former assassin, known only as The Bride, who continues seeking vengeance against her ex-lover, Bill, and his deadly squad of international assassins. Betrayed and left for dead on her wedding day by the very group she once considered family, The Bride survives and sets out on a mission of retribution. Having already dispatched two members of the team, she turns her attention to the remaining targets: Bill's brother Bud, a bouncer living in a trailer, and Elle Driver, a one-eyed killer with whom The Bride shares a fraught history.

As The Bride journeys through this perilous path of vengeance, the narrative unveils more of her backstory with Bill, exploring the depth of their past relationship and the reasons behind her brutal betrayal. The film delves into the origins of The Bride's training, her skills honed under the tutelage of a martial arts master, and the complex dynamics within the assassin organization. The Bride’s quest not only pits her against formidable foes but also forces her to confront the moral complexities of her past actions and the inexorable cycle of violence she's entangled in.