In the plot, a struggling songwriter named Dave Seville finds his life turned upside down when he crosses paths with three talking chipmunks named Alvin, Simon, and Theodore. These chipmunks are not ordinary animals; they possess exceptional singing talents. Initially, Dave is unaware of the chipmunks' unique abilities, but he soon discovers their musical talent when he hears them sing. Seeing an opportunity to turn his fortunes around, Dave decides to take them in and work with them. He dreams of making it big in the music industry by producing records featuring the chipmunks' singing.

As Dave starts to integrate the chipmunks into his life, he faces the challenges of managing the trio's rising fame while trying to maintain a normal life. The chipmunks' presence brings about a series of comedic and heartwarming incidents, particularly as they adapt to human society and unintentionally wreak havoc in Dave's daily routine. The relationship between Dave and the chipmunks deepens as they work together, navigating the complexities of fame and success. The plot explores themes of family, ambition, and the highs and lows of the entertainment industry, showcasing the journey of an unlikely quartet striving to achieve their dreams amidst various obstacles.