In London, a Dalmatian named Pongo lives with his human, Roger, a bachelor songwriter. Pongo decides that both he and Roger need female companionship, so he orchestrates a meeting with a lovely Dalmatian named Perdita and her owner, Anita. The plan works, and soon Roger and Anita are married, with Pongo and Perdita happily sharing their home. Perdita gives birth to 15 puppies, filling their household with joy. However, their delight is short-lived when Anita's former schoolmate, the fashion-obsessed Cruella de Vil, becomes infatuated with the puppies and schemes to acquire them for a lavish fur coat. After her offer to buy the puppies is refused, the devious Cruella engages two henchmen, Jasper and Horace, to kidnap the pups.

Amidst panic and worry, Pongo and Perdita set out on a daring mission to rescue their litter, guided by the Twilight Barking—a network of communication among dogs. Their journey leads them to Cruella's secluded manor house, Hell Hall, where they discover not only their own puppies but also many other Dalmatian puppies Cruella has stolen. What follows is a perilous and heart-pounding adventure as Pongo and Perdita strive to save the stolen pups while evading Cruella and her henchmen. The story unfolds with thrilling twists and turns as the brave Dalmatians work together to bring all the puppies to safety.