The Mystery Inc. gang—Scooby-Doo, Shaggy, Fred, Daphne, and Velma—ventures to a KISS-themed amusement park to attend a special Halloween concert by the iconic rock band KISS. When they arrive, they discover that the park is under threat from a spooky and mysterious Crimson Witch who seeks to unleash a menacing evil entity called The Destroyer. KISS is already investigating the strange occurrences, and they team up with the gang to get to the bottom of the mystery. The Crimson Witch's eerie antics create chaos, leading to the possibility of the concert being canceled if the mystery is not solved.

Throughout their adventure, both groups uncover clues that point toward an ancient prophecy linked to KISS's superhero alter-egos and special powers. They navigate through various park attractions and haunted areas, piecing together information while dodging supernatural threats. With time running out before The Destroyer can be summoned, the team must unearth critical secrets and collaborate effectively. This leads them on a thrilling ride filled with musical performances, magical transformations, and high-energy chases as they work to save the park and foil the Crimson Witch's plans.