In a vibrant, fantastical realm, two Brooklyn plumbers named Mario and Luigi unexpectedly find themselves pulled into a magical pipe during a routine job. Emerging in the mystical Mushroom Kingdom, they quickly realize they're far from home. The kingdom is under threat from the tyrannical Bowser, a fearsome Koopa king who has nefarious plans for conquest. Separated from his brother, Mario teams up with Princess Peach, the kingdom’s determined leader, and Toad, a loyal citizen of the Mushroom Kingdom. They embark on a daring quest across various whimsical and treacherous landscapes in an urgent mission to locate Luigi and thwart Bowser’s plans.

Along their journey, they encounter numerous allies and adversaries, each contributing unique challenges and assistance. Guided by Peach's leadership and Mario’s evolving bravery, they form a formidable team against Bowser’s minions. Their adventures test their courage, wit, and resilience, leading to self-discovery and forging deep bonds of friendship. As Mario and Peach navigate through the kingdom's intricate challenges, they uncover hidden strengths while preparing for an inevitable confrontation with Bowser. With time running out and stakes reaching their peak, their journey grows ever more perilous, setting the stage for an epic showdown to decide the fate of the Mushroom Kingdom.