In the late 21st century, a team of scientists and explorers undertakes a journey aboard the spacecraft named Prometheus, funded by the powerful Weyland Corporation. Their mission is based on ancient star maps discovered on Earth, which point to a distant moon, LV-223, that house the potential to unlock humanity's greatest mystery: the origins of human life. Leading the expedition are archaeologist Dr. Elizabeth Shaw and her partner Dr. Charlie Holloway, who are driven by the possibility of meeting humanity's creators, who they refer to as the "Engineers."

Upon arrival at LV-223, the crew of Prometheus finds an ancient alien structure that appears to be a temple or military installation but contains cryogenic pods. Here, they hope to find answers about the Engineers. The team's ambitious endeavor and curiosity lead to a series of increasingly disturbing discoveries. David, an enigmatic and advanced android that accompanies the crew, explores the alien environment and interacts with its technology, demonstrating an agenda that aligns with, yet diverges from, the human members of the crew. Tension rises as the true nature of the mission becomes uncertain, forcing the team to question not only their purpose but if they are truly prepared for the consequences of unveiling secrets best left hidden.