Blu and Jewel, along with their three children, are now living in the bustling city of Rio de Janeiro. Their human friends, Linda and Tulio, discover evidence that suggests there might be other blue macaws living deep in the Amazon. Determined to find these birds and show their kids what it means to live like real birds, Jewel persuades Blu to embark on a journey to the Amazon. As they navigate through the jungle, Blu struggles to fit in and prove himself as a real bird while also dealing with his fear of the wild.

Upon arriving in the Amazon, they encounter a hidden tribe of blue macaws, which includes Jewel's long-lost father. As Blu tries to win over his skeptical father-in-law while contending with new threats from the encroaching humans and a vengeful old enemy from Rio, he must find a balance between his domesticated ways and the demands of jungle life.