Greg Focker and his fiancée Pam Byrnes decide to introduce their respective parents before their upcoming wedding. Pam's conservative and ex-CIA agent father, Jack Byrnes, and her mother, Dina, agree to meet Greg's free-spirited parents, Bernie and Roz Focker. The Byrnes family, with their strict and traditional values, embark on a road trip in their RV to meet the Fockers in their laid-back, colorful home in Florida. Jack, highly suspicious and overprotective, insists on digging into every aspect of Greg's background, including putting the Focker household under scrutiny.

The contrasting parenting styles and lifestyles immediately cause tension between the two families. Jack's rigid discipline clashes with Bernie’s affectionate and whimsical demeanor, while Roz, a sex therapist, shocks the reserved Byrnes family with her openness. Greg finds himself caught in the middle, trying to mediate and prevent any further mishaps. As Jack continues to investigate Greg’s past, misunderstandings and comedic situations escalate, pushing the tensions within both families to a boiling point.