In a colorful and exuberant world populated by the ever-singing and dancing Trolls, a new adventure begins when an unexpected threat looms over their harmonious way of life. The Trolls discover that their music, the very heart of their culture, is at risk of being silenced forever by a mysterious new enemy. With their vibrant way of life in jeopardy, the community's leaders, Poppy and Branch, realize they cannot face this challenge alone and must seek the help of other Troll tribes—each dedicated to a different genre of music.

To unite the Trolls against the looming threat, Poppy and Branch set out on an epic journey that takes them far beyond the only world they've ever known. They venture out to seek the aid of the scattered Troll tribes: the Funk, Country, Techno, Classical, and Rock Trolls, among others. Along the way, they encounter a diverse cast of characters, each bringing their unique musical talents, perspectives, and solutions to the table. The Trolls must band together and embrace their differences, combining their distinct musical styles against the common threat, learning the true power of harmony and diversity in the process.