Following the events of its predecessor, humanity is on the brink of extinction due to the T-virus unleashed by the sinister Umbrella Corporation. Alice, the former Umbrella employee turned rogue warrior, emerges as humanity's last hope. She finds herself in the devastated landscape of Washington D.C., where she must gather her strength and wits to embark on a perilous journey. Aided by unexpected allies, she is driven by a message from the Red Queen, offering a chance to end the apocalypse once and for all.

Alice's mission leads her back to where the nightmare began: The Hive in Raccoon City, where Umbrella is preparing for a final strike against the remaining survivors. En route, she must confront various bio-engineered obstacles and former friends turned foes. The stakes reach unimaginable heights as she uncovers more about her past and the true motives of Umbrella. As the countdown to humanity's fate ticks on, Alice stands as the beacon of resistance in a world overrun by the undead and mutated creatures, determined to stop the virus before it is too late.