The story begins with Woody, Buzz Lightyear, Jessie, and the rest of the gang embarking on a road trip with their new owner, Bonnie. During their journey, Bonnie and her mother have to make an unexpected stop at a roadside motel for the night. As the toys settle in, they soon realize that the motel holds darker secrets when Mr. Potato Head mysteriously disappears. The toys set out to find him, leading them through eerie corridors and spooky rooms, filled with unexpected traps and clues that suggest foul play.

As the search intensifies, Woody, Buzz, and Jessie uncover that their friends are being captured by the motel's nefarious manager and his pet iguana for a sinister purpose. Together, they must hatch a plan to save their friends while facing their own fears in the process. As Jessie struggles with her claustrophobia, her bravery is put to the test in an adventure filled with suspense and excitement. The atmosphere grows even more tense as their time starts to run out.