In New York City, beneath the bustling streets, four unconventional brothers emerge from the sewers. They're not your typical teenagers; they're a quartet of genetically mutated turtles with a penchant for martial arts and an unbreakable bond as brothers. Named after Renaissance artists, Leonardo, Raphael, Donatello, and Michelangelo were raised and trained by their rat sensei, Splinter. Hidden from the world, they've grown up on a heavy diet of pizza and brotherhood, honing their skills and forming a vigilant team under the cover of darkness.

The turtles' routine life is upended when a surge of strange, mutated creatures start wreaking havoc on the city. Alongside this chaos, the existence of mutants becomes widely known, leading to fear and unrest among the citizens. Eager to prove themselves as heroes and win the acceptance of the public, the brothers venture out regularly to fight crime and face the rising tide of evil. During their adventures, they encounter a tenacious reporter, April O'Neil, and a host of other allies and adversaries. The turtles must navigate the complexities of friendship, trust, and the clash between the desire for open lives versus the safety of secrecy. Meanwhile, a sinister figure with a personal vendetta emerges, challenging their efforts to protect the city and posing a threat that may unravel the fabric of their very existence.